ea0029oc13.2 | Adrenal Basic | ICEECE2012
Monticone S.
, Crudo V.
, Burrello J.
, Galmozzi M.
, Warth R.
, Veglio F.
, Mulatero P.
, Williams T.
Visinin-like 1 (VSNL1) is upregulated in aldosterone-producing adenomas (APA) compared to normal adrenals. We demonstrate that VSNL1 overexpression in adrenocortical carcinoma cells (NCI H295R) upregulates basal and angiotensin II (Ang II)-stimulated CYP11B2 gene expression 3.2- and 1.5-fold, respectively. Conversely, silencing VSNL1 by RNA interference decreases Ang II-stimulated CYP11B2 expression and aldosterone secretion by 41 and 34.5%, respectively. Mutations in the pota...